Journal: bioRxiv
Article Title: Exposure to third generation cephalosporin induces L-form transition in Shigella sonnei , potentially acting as a bacterial reservoir for persistent infection
doi: 10.1101/2025.07.17.665312
Figure Lengend Snippet: (A) Circular map of the 4.57 Mb chromosome of S. sonnei HK8. The concentric rings, from the outside in, represent: (1-2) COG functional categories and coding sequences (CDS) on the positive strand; (3) RNA genes; (4-5) CDS and COG categories on the negative strand; (6) GC content relative to the mean; and (7) GC skew [(G-C)/(G+C)]. (B) Whole-genome phylogenomic tree illustrating the evolutionary position of S. sonnei HK8. The analysis, performed using the Type Strain Genome Server (TYGS), places HK8 (highlighted in red) directly adjacent to the Shigella sonnei ATCC 29930 type strain, confirming its species identity. The tree scale indicates the evolutionary distance. (C) Bar chart showing the absolute counts of putative virulence-associated genes in the HK8 genome, categorized by confidence level. The analysis identified a large number of genes with homology to “Potential Non-secreted Virulence factors” and a substantial fraction classified as “Unknown.” Genes with stronger evidence were categorized as “Non-secreted Virulence factors,” “Secreted Virulence factors,” and “Potential Secreted Virulence factors,” providing a quantitative overview of the isolate’s pathogenic potential.
Article Snippet: For comprehensive genome-based phylogenomic analysis and strain-level classification, the assembled genome was submitted to the Type Strain Genome Server (TYGS) ( https://tygs.dsmz.de/ ).
Techniques: Functional Assay